For further information and operational details, please contact: Aaron Barlow
Leica Upright Fluorescence Microscope with Fast Confocal Scanner and CCD Camera
This is a filter-free spectral confocal and multiphoton microscope equipped with SP2 RS AOBS laser scan head which is attached to a Leica DM LFSA upright microscope. The imaging operations which include selections of laser, detection channels and other functions are fully automated and computer controlled. Many of its notable features are listed below.
Notable Features:
- Fully automated operational procedures
- High experimental throughput
- Dipping high numerical aperture objectives for in-vivo imaging
- Multi-photon and visible confocal in one system
- Ultra-short pulse infrared laser for multi-photon imaging ( See details below )
- Non de-scanned detectors for the purpose of multi-photon and SHG imaging
- Transmission light detector for the transmission imaging
- Near video rate imaging capabilities with simultaneous or sequential scanning options
- Specially designed swing-out stage for mounting large specimens
- AOBS selection and slit discrimination of collected emission allows for scroll bar style selection of band-width, from as little as a 5 nm window, to several hundred nm. This feature allows determination of fluorophore emission intensity profiles in frequency space
- Complex patterns of line and area scanning, 3-dimensional series, time series, wavelengthseries, and FRAP, FRET measurements
- Comprehensive image processing functions